Laser capture microdissection (LCM) and RNA isolation
نویسندگان
چکیده
منابع مشابه
Single-neuron isolation for RNA analysis using pipette capture and laser capture microdissection.
The field of single-cell analysis has greatly benefitted from recent technological advances allowing scientists to study genomes, transcriptomes, proteomes, and metabolomes at the single-cell level. Transcriptomics allows a unique window into cell function and is especially useful for studying global variability among single cells of seemingly the same type. Generating transcriptome data from R...
متن کاملLaser-capture microdissection.
Laser-capture microdissection is a recently discovered state-of-the-art method to obtain cells for genetic analysis. It is a one-step procedure that allows capture of selected cells under direct microscopic visualization.
متن کاملRNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling.
Laser capture microdissection (LCM) allows the isolation of specific cells from thin tissue sections with high spatial resolution. Effective LCM requires precise identification of cells subpopulations from a heterogeneous tissue. Identification of cells of interest for LCM is usually based on morphological criteria or on fluorescent protein reporters. The combination of LCM and rapid immunolabe...
متن کاملLaser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions
Macrophage foam cells are integral in the development of atherosclerotic lesions, however gene expression studies are complicated by the cellular heterogeneity of atherosclerotic plaque. This application note describes a protocol for LCM of cells identifi ed immunohistochemically, followed by realtime RT-PCR to selectively analyze RNA from foam cells of apolipoprotein E-defi cient mice. The spe...
متن کاملLaser Capture Microdissection in Dentistry
Laser capture microdissection (LCM) allows for the microscopic procurement of specific cell types from tissue sections that can then be used for gene expression analysis. According to the recent development of the LCM technologies and methodologies, the LCM has been used in various kinds of tissue specimens in dental research. For example, the real-time polymerase-chain reaction (PCR) can be pe...
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ژورنال
عنوان ژورنال: Protocol Exchange
سال: 2013
ISSN: 2043-0116
DOI: 10.1038/protex.2013.027